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Atlas of Computational Cell Reprogramming

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L3 · Explicit model-based inverse intervention D P

NETISCE

Marazzi L, Shah M, Balakrishnan S, Patil A, Vera-Licona P

2022 · NPJ systems biology and applications

The search for effective therapeutic targets in fields like regenerative medicine and cancer research has generated interest in cell fate reprogramming.

Abstract

From the original paper, NPJ systems biology and applications · PubMed

The search for effective therapeutic targets in fields like regenerative medicine and cancer research has generated interest in cell fate reprogramming. This cellular reprogramming paradigm can drive cells to a desired target state from any initial state. However, methods for identifying reprogramming targets remain limited for biological systems that lack large sets of experimental data or a dynamical characterization. We present NETISCE, a novel computational tool for identifying cell fate reprogramming targets in static networks. In combination with machine learning algorithms, NETISCE estimates the attractor landscape and predicts reprogramming targets using signal flow analysis and feedback vertex set control, respectively. Through validations in studies of cell fate reprogramming from developmental, stem cell, and cancer biology, we show that NETISCE can predict previously identified cell fate reprogramming targets and identify potentially novel combinations of targets. NETISCE extends cell fate reprogramming studies to larger-scale biological networks without the need for full model parameterization and can be implemented by experimental and computational biologists to identify parts of a biological system relevant to the desired reprogramming task.

Summary

Editorial summary pending review by the maintainer. The paper's own abstract appears above; the Atlas summary in the maintainer's voice will explain how NETISCE relates to the cross-modality inverse-design framework of the review.

Why this level

Level 3 because candidate interventions enter an explicit forward operator FθuF_{\theta_u} and the predicted post-intervention outcome is what scores each candidate. Representation family is executable intervention model. Cited in §3.4 of the review. Editorial rationale pending review by the maintainer.

Classification

Level
L3
Representation
Executable intervention model
Modalities
D, P
Intervention
Transcription factors
Framework
Boolean network

Software

Reproducibility
4 of 4
FAIR4RS
3 of 5

Last audited 2026-05-24

Citation

Marazzi L et al. (2022). NETISCE: a network-based tool for cell fate reprogramming., NPJ systems biology and applications.

DOI: 10.1038/s41540-022-00231-y

PMID: 35725577

BibTeX
@article{netisce2022,
  title  = {NETISCE: a network-based tool for cell fate reprogramming.},
  author = {Marazzi L et al.},
  year   = {2022},
  journal = {NPJ systems biology and applications},
  pmid = {35725577},
  doi  = {10.1038/s41540-022-00231-y}
}